8CQP
Crystal structure of an 8-repeat consensus TPR superhelix with Calcium (low concentration)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.448, 70.249, 102.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.970 - 1.700 |
R-factor | 0.20311 |
Rwork | 0.201 |
R-free | 0.23897 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.545 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.250 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmeas | 0.101 | 1.392 |
Rpim | 0.041 | 0.571 |
Total number of observations | 310018 | 44469 |
Number of reflections | 53530 | 7724 |
<I/σ(I)> | 7.9 | 1.4 |
Completeness [%] | 99.9 | |
Redundancy | 5.8 | 5.8 |
CC(1/2) | 0.995 | 0.477 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | CaCl2, 10 mM 100 mM NaOAc, pH 5.5 30% MPD |