8CQH

Ntaya virus methyltransferase in complex with GTP and SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-23
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541870
Spacegroup name	P 1 21 1
Unit cell lengths	38.250, 71.655, 50.413
Unit cell angles	90.00, 93.15, 90.00

Refinement procedure

Resolution	27.400 - 2.000
R-factor	0.154
Rwork	0.152
R-free	0.18980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.400	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	18427	1817
<I/σ(I)>	22.1	7.2
Completeness [%]	99.9
Redundancy	13.3
CC(1/2)	0.998	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Trizma/Bicine pH 8.5, 0.02M monosacharides, 10% (w/v) PEG4000, 20% glycerol