8CQ7
Crystal structure of phyllanthoside bound to the Candida albicans 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2021-06-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 299.770, 294.170, 451.170 |
Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
Resolution | 228.180 - 3.200 |
R-factor | 0.2466 |
Rwork | 0.246 |
R-free | 0.28110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.792 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 228.180 | 228.180 | 3.250 |
High resolution limit [Å] | 3.200 | 50.000 | 3.200 |
Rmerge | 0.736 | 0.093 | 3.471 |
Rmeas | 0.753 | 0.096 | 3.552 |
Number of reflections | 1261990 | 296 | 57141 |
<I/σ(I)> | 4.7 | ||
Completeness [%] | 100.0 | ||
Redundancy | 21.5 | ||
CC(1/2) | 0.994 | 0.996 | 0.069 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside |