8CP7

Structure of the disulfide-locked substrate binding protein HiSiaP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.826554
Spacegroup name	I 2 2 2
Unit cell lengths	84.083, 89.451, 90.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.040 - 1.900
R-factor	0.2127
Rwork	0.210
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.033
Phasing software	PHENIX (dev_4788)
Refinement software	PHENIX (dev_4788)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.040	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.029	0.613
Number of reflections	26990	2437
<I/σ(I)>	14.84
Completeness [%]	99.0
Redundancy	2
CC(1/2)	1.000	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.01 M Zinc chloride, 0.1 M Sodium acetate, pH 5.0, 20% w/v PEG 6000