8CP3
Apo-LarE in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | H 3 2 |
Unit cell lengths | 144.810, 144.810, 193.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.160 - 2.350 |
R-factor | 0.2327 |
Rwork | 0.232 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.095 |
Data reduction software | XDS (Mars, 2021) |
Data scaling software | XDS (Mars, 2021) |
Phasing software | MOLREP (Mars, 2021) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.180 | 48.180 | 2.490 |
High resolution limit [Å] | 2.350 | 6.990 | 2.350 |
Rmeas | 0.067 | 0.028 | 2.432 |
Number of reflections | 32562 | 1325 | 5128 |
<I/σ(I)> | 25.46 | 98.39 | 0.95 |
Completeness [%] | 99.7 | 99.5 | 98.5 |
Redundancy | 20.7 | ||
CC(1/2) | 1.000 | 1.000 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.6 M Ammonium Sulfate, 2 % PEG 400, 0.1 M Hepes pH 7.5, 5 mM AMP-PNP. |