8CP3
Apo-LarE in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.810, 144.810, 193.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.160 - 2.350 |
| R-factor | 0.2327 |
| Rwork | 0.232 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS (Mars, 2021) |
| Data scaling software | XDS (Mars, 2021) |
| Phasing software | MOLREP (Mars, 2021) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.180 | 48.180 | 2.490 |
| High resolution limit [Å] | 2.350 | 6.990 | 2.350 |
| Rmeas | 0.067 | 0.028 | 2.432 |
| Number of reflections | 32562 | 1325 | 5128 |
| <I/σ(I)> | 25.46 | 98.39 | 0.95 |
| Completeness [%] | 99.7 | 99.5 | 98.5 |
| Redundancy | 20.7 | ||
| CC(1/2) | 1.000 | 1.000 | 0.501 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.6 M Ammonium Sulfate, 2 % PEG 400, 0.1 M Hepes pH 7.5, 5 mM AMP-PNP. |
