8COH

Structure of the complement C5 specific nanobody TPP-3444

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	81.489, 81.489, 36.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.440 - 1.300
R-factor	0.1481
Rwork	0.146
R-free	0.17460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.440	1.347
High resolution limit [Å]	1.300	1.300
Rmerge	0.107	1.447
Number of reflections	30885	3019
<I/σ(I)>	22.55
Completeness [%]	99.9	99.7
Redundancy	25.9	25
CC(1/2)	1.000	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.1	277	54 mM citric acid, 36 mM BIS-TRIS propane pH 4.1, 14.4% w/v polyethylene glycol 3350, and 10 mM NiCl2.