8COB
Crystal structure of human PCNA in complex with ERCC6L2 PIP box peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-28 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.96600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.694, 109.082, 139.396 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.560 - 2.730 |
R-factor | 0.1999 |
Rwork | 0.197 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mlo |
RMSD bond length | 0.007 |
RMSD bond angle | 1.139 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3126) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.560 | 2.860 |
High resolution limit [Å] | 2.730 | 2.730 |
Rmerge | 0.118 | 0.531 |
Rmeas | 0.133 | 0.599 |
Rpim | 0.059 | 0.272 |
Number of reflections | 28128 | 3678 |
<I/σ(I)> | 10.5 | 3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5 | 4.8 |
CC(1/2) | 0.995 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% (w/v) PEG3350, 100 mM BIS-TRIS pH 5.5 |