8CNT
Structure of the DEAH-box helicase Prp16 in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976250 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.130, 102.140, 106.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.970 - 1.900 |
R-factor | 0.19527 |
Rwork | 0.193 |
R-free | 0.23819 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.486 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.970 | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 17.000 | 1.900 |
Rmerge | 0.106 | 0.039 | 1.562 |
Rmeas | 0.115 | 0.044 | 1.686 |
Number of reflections | 91538 | 123 | 13012 |
<I/σ(I)> | 11.78 | ||
Completeness [%] | 99.9 | ||
Redundancy | 7.07 | ||
CC(1/2) | 0.999 | 0.998 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5 0.005 M Magnesiumchloride 20 % PEG 4000 (w/v) |