8CMQ
Crystal structure of an 8-repeat consensus TPR superhelix with Tb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.650042 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.769, 57.485, 76.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.910 - 1.782 |
| R-factor | 0.17459 |
| Rwork | 0.173 |
| R-free | 0.19767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.634 |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.913 | 1.812 |
| High resolution limit [Å] | 1.782 | 1.782 |
| Rmerge | 0.152 | 1.720 |
| Rmeas | 0.159 | 1.808 |
| Rpim | 0.046 | 0.550 |
| Number of reflections | 18929 | 926 |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 11.9 | 10.5 |
| CC(1/2) | 0.993 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 292 | TbCl3, 5 mM 100 mM Tris-HCl 30% MPD |
