8CMN

18mer DNA mimic Foldamer with an aliphatic linker in complex with Sac7d wild protein

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	77.36
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.8856
Spacegroup name	P 64 2 2
Unit cell lengths	71.410, 71.410, 116.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.340 - 2.650
R-factor	0.2651
Rwork	0.261
R-free	0.29740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	2.686
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.340	2.750
High resolution limit [Å]	2.650	2.650
Number of reflections	5508	531
<I/σ(I)>	18.81
Completeness [%]	99.6
Redundancy	6.9
CC(1/2)	0.998	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	10% PEG 400, 0.1 M MES pH 6.0