8CMK
Transportin-3 TNPO3 in complex with RSY region of CIRBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-05 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.033220 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.526, 101.805, 114.133 |
Unit cell angles | 90.00, 111.13, 90.00 |
Refinement procedure
Resolution | 48.715 - 2.945 |
Rwork | 0.246 |
R-free | 0.31660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.577 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.720 | 48.720 | 3.060 |
High resolution limit [Å] | 2.940 | 11.020 | 2.940 |
Rmerge | 0.094 | 0.044 | 0.672 |
Rmeas | 0.130 | 0.061 | 0.923 |
Rpim | 0.090 | 0.043 | 0.629 |
Number of reflections | 43555 | 876 | 4458 |
<I/σ(I)> | 8 | ||
Completeness [%] | 98.5 | ||
Redundancy | 3.5 | 3.3 | 3.5 |
CC(1/2) | 0.996 | 0.995 | 0.509 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.5ul Protein (TNPO3) 12mg/ml with 0.2ul Ligand (CIRBP) 4.4mg/ml and 0.1ul seeding stock were mixed with 0.5ul of condition. Condition: 0.1M Sodium HEPES, 0.1M MOPS (acid), 7.5pH, 12.5% v/v MPD; 12.5% PEG 1000; 12.5% w/v PEG 3350, 0.05% w/v D-Salicin, 0.05% w/v Esculin hydrate, 0.05% w/v Quinine hemisulfate salt monohydrate, 0.05% w/v Tryptamine, 0.05% w/v Arbutin (in 50% EtOH) Protein and Ligand in 50mM Tris, 150mM NaCl, 2mM TCEP, 0.04% NaN3, pH: 7.5 Buffer |