8CM1

Lol B - Localization of lipoprotein B from Vibrio cholera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97624
Spacegroup name	P 41 21 2
Unit cell lengths	113.414, 113.414, 34.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.860 - 1.460
R-factor	0.1673
Rwork	0.166
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.955
Data reduction software	autoPROC (1.20.1-4487)
Data scaling software	autoPROC (1.20.1-4487)
Phasing software	PHENIX (1.19.2-4158)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.100	1.512
High resolution limit [Å]	1.460	1.460
Rmerge	0.127	0.374
Rmeas	0.132	0.529
Rpim	0.026	0.374
Number of reflections	39405	3862
<I/σ(I)>	19.6	1.69
Completeness [%]	100.0	99.97
Redundancy	2
CC(1/2)	0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291.15	0.1M KCl, 0.1 M Bis-Tris 6.0, 18% w/v PEG4000