8CM0
Rhs2-CT endonuclease toxin in complex with cognate immunity protein RhsI2
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-09 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.006, 81.931, 101.371 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.980 - 2.170 |
Rwork | 0.195 |
R-free | 0.25260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.477 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASES |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.980 | 2.240 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.157 | 1.511 |
Rpim | 0.070 | 0.661 |
Number of reflections | 17007 | 1408 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6 | |
CC(1/2) | 0.993 | 0.542 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | Crystals were grown in a condition containing 0.2 uL of 6.5 mg/mL Rhs2CT-RhsI2 (in 20 mM MES & 20 mM imidazole pH 6.0, 150 mM NaCl, 1 mM MgCl2, 0.5 mM TCEP) and 0.2 uL of reservoir solution (0.2 M ammonium acetate, 0.1 M sodium acetate pH 5.0, 20% PEG4K). |