8CLZ

Crystal structure of Rhizobium etli constitutive L-asparaginase ReAIV (monoclinic form R4mC-2)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9770
Spacegroup name	C 1 2 1
Unit cell lengths	96.245, 85.298, 82.946
Unit cell angles	90.00, 109.18, 90.00

Refinement procedure

Resolution	78.340 - 1.501
Rwork	0.253
R-free	0.30240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.479
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.340	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.088	0.849
Rmeas	0.107	1.044
Number of reflections	100056	16066
<I/σ(I)>	6.6	1.12
Completeness [%]	99.1
Redundancy	2.99
CC(1/2)	0.994	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium formate, 20 % (w/v) PEG 3350, and 0.2% lauryldimethylamine oxide (LDAO)