8CLW
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (CKO/CKX4) in complex with inhibitor 2-[(3,5-dichlorophenyl)carbamoylamino]-4-methoxy-benzamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.991870 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.560, 79.560, 203.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.280 - 1.800 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | XDS (BUILT=20180307) |
Data scaling software | XDS (BUILT=20180307) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.300 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmeas | 0.063 | 1.253 |
Rpim | 0.024 | 0.604 |
Number of reflections | 60808 | 9086 |
<I/σ(I)> | 15.7 | 0.9 |
Completeness [%] | 98.8 | 93 |
Redundancy | 6.4 | 3.6 |
CC(1/2) | 1.000 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 21% MPD, 1 mM inhibitor and protein at concentration of 10 mg ml-1 in the starting drop |