8CLT

Zearalenone lactonase of Rhodococcus erythropolis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976250
Spacegroup name	C 2 2 21
Unit cell lengths	64.467, 121.803, 172.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.530 - 1.460
R-factor	0.165
Rwork	0.164
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.530	1.512
High resolution limit [Å]	1.460	1.460
Rmerge	0.101	4.677
Rmeas	0.111	5.527
Rpim	0.044	2.872
Number of reflections	114638	9488
<I/σ(I)>	10.2	0.18
Completeness [%]	93.0	39.33
Redundancy	6	0.18
CC(1/2)	0.999	0.109

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295.15	0.2 M NaCl, 0.1 M BIS-TRIS 5.5 pH, 25% w/v PEG 3350