8CLP

Zearalenone lactonase from Streptomyces coelicoflavus mutant H286Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	63.569, 126.128, 128.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.570 - 1.920
R-factor	0.1986
Rwork	0.197
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.506
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4489)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.570	1.989
High resolution limit [Å]	1.920	1.920
Rmerge	0.110	0.766
Rmeas	0.128	0.908
Rpim	0.064	0.471
Number of reflections	76545	7561
<I/σ(I)>	8.22	1.34
Completeness [%]	95.9	95.56
Redundancy	3.3	3.2
CC(1/2)	0.996	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	295.15	0.1 M Mg formate dihydrate, 15% PEG 3350 (pH 6.9)