8CLM

1.80 A crystal structure of RNA/2'-O-methyl-RNA heteroduplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-12
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 1 21 1
Unit cell lengths	30.103, 49.303, 55.868
Unit cell angles	90.00, 97.15, 90.00

Refinement procedure

Resolution	19.011 - 1.800
Rwork	0.188
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.952
Data reduction software	CrysalisPro (41.100a)
Data scaling software	Aimless (0.7.7)
Phasing software	MOLREP (11.7.03)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.011	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.081	0.636
Rmeas	0.094	0.747
Rpim	0.046	0.382
Number of reflections	15124	868
<I/σ(I)>	11.7	2.1
Completeness [%]	99.7	100
Redundancy	4.2	3.6
CC(1/2)	0.998	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1 mM duplex, 1.4 M Li2SO4, 50 mM sodium cacodylate, 1 mM spermine