8CLC
Tubulin (T2R-TTL) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SwissFEL ARAMIS BEAMLINE ESA |
Synchrotron site | SwissFEL ARAMIS |
Beamline | ESA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2020-02-28 |
Detector | PSI JUNGFRAU 16M |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.530, 160.480, 181.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.200 - 2.700 |
R-factor | 0.1868 |
Rwork | 0.186 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.462 |
Data reduction software | CrystFEL (0.8.0) |
Data scaling software | CrystFEL (0.8.0) |
Phasing software | PHENIX (1.20_4487) |
Refinement software | PHENIX (1.20_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.220 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 121578 | 12016 |
<I/σ(I)> | 3.88 | 0.41 |
Completeness [%] | 100.0 | |
Redundancy | 185.5 | |
CC(1/2) | 0.951 | 0.190 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.5 | 293 | 6% PEG4000 (w/v), 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |