8CL9

Tubulin-DARPin D1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SwissFEL ARAMIS BEAMLINE ESA
Synchrotron site	SwissFEL ARAMIS
Beamline	ESA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-02-28
Detector	PSI JUNGFRAU 16M
Wavelength(s)	1.03
Spacegroup name	P 1 21 1
Unit cell lengths	73.470, 91.190, 82.700
Unit cell angles	90.00, 96.43, 90.00

Refinement procedure

Resolution	13.000 - 2.500
R-factor	0.1879
Rwork	0.185
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	CrystFEL (0.8.0)
Data scaling software	CrystFEL (0.8.0)
Phasing software	PHASER (1.20_4459)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.940	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	63162	6206
<I/σ(I)>	3.58	0.52
Completeness [%]	100.0
Redundancy	71.9
CC(1/2)	0.931	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	5.5	293	21% PEG3000 (w/v), 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris Methane