8CL3
HIV-1 mature capsid hexamer from CA-IP6 CLPs, bound to Sec24C peptide.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.14 |
| Number of Particle | 2464856 |
| Software | crYOLO,cryoSPARC,ISOLDE,Coot,PHENIX,EPU,UCSF Chimera |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
