8CKT
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (CKO/CKX4) in complex with inhibitor 2-[(3,5-dichlorophenyl)carbamoylamino]-4-(trifluoromethoxy)benzamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980113 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.250, 79.250, 204.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.270 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS (BUILT=20220220) |
| Data scaling software | XDS (BUILT=20220220) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.213 | 3.238 |
| Rpim | 0.041 | 0.614 |
| Number of reflections | 44973 | 7132 |
| <I/σ(I)> | 16.1 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26.7 | 27.1 |
| CC(1/2) | 0.999 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 22% MPD, 1 mM inhibitor, and protein at 10 mg ml-1 concentration in the starting drop. |
