8CKR

Crystal structure of an 8-repeat consensus TPR superhelix with in Hepes with Ca

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 32 2 1
Unit cell lengths	68.317, 68.317, 72.335
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.160 - 1.300
R-factor	0.18741
Rwork	0.187
R-free	0.19866
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.758
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (7.0.078)
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.170	1.370
High resolution limit [Å]	1.300	1.300
Rmeas	0.087	2.322
Rpim	0.022	0.605
Total number of observations	729150	101260
Number of reflections	48472	6993
<I/σ(I)>	11	1.3
Completeness [%]	100.0
Redundancy	15	14.5
CC(1/2)	0.999	0.438

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	292	20mM CaCl2 100 mM Hepes 30% MPD