8CKQ
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (CKO/CKX4) in complex with inhibitor 2-[(3,5-dichlorophenyl)carbamoylamino]benzamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.980109 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.470, 79.470, 203.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS (BUILT=20190315) |
| Data scaling software | XDS (BUILT=20190315) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.133 | 2.764 |
| Rpim | 0.028 | 0.570 |
| Number of reflections | 45188 | 7101 |
| <I/σ(I)> | 15.4 | 1.3 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 22.2 | 22.4 |
| CC(1/2) | 0.998 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% MPD in 50 mM HEPES, pH 7.5, 1.4 mM inhibitor and protein at 9.7 mg ml-1 concentration in the starting drop |
