8CK7
Avidin complexed to theophylline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2013-02-13 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 64 |
Unit cell lengths | 108.910, 108.910, 43.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.500 - 2.200 |
R-factor | 0.18639 |
Rwork | 0.184 |
R-free | 0.23332 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.662 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.500 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 15151 | 1308 |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.1 | |
CC(1/2) | 0.980 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-Tris-Propane pH 8.5, 1.7-2.3 M w/v Ammonium Sulfate, 1-5% v/v MPD |