8CJV
Structure of bovine CD46 ectodomain (SCR 1-4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-02 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 129.731, 129.731, 120.193 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.170 - 2.840 |
R-factor | 0.2543 |
Rwork | 0.253 |
R-free | 0.28550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.840 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.730 | 3.240 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 0.487 | 2.225 |
Rmeas | 0.497 | 2.278 |
Rpim | 0.097 | 0.473 |
Number of reflections | 10884 | 544 |
<I/σ(I)> | 7.1 | 1.8 |
Completeness [%] | 93.3 | |
Redundancy | 25.6 | 20.5 |
CC(1/2) | 0.998 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 10.5mg/ml in 0.1M sodium cacodylate tetrahydrate pH 6.4, 14% PEG smear broad, 0.2M CaCl2, 5% glycerol . |