8CJ4
Crystal structure of ClpP from Staphylococcus epidermidis, tetradecamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.423, 123.662, 126.787 |
| Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
| Resolution | 44.960 - 1.900 |
| R-factor | 0.1829 |
| Rwork | 0.182 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.960 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.090 | 0.940 |
| Number of reflections | 227961 | 22466 |
| <I/σ(I)> | 14.12 | 2.14 |
| Completeness [%] | 98.7 | 97.58 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298.15 | 0.1M sodium acetate (pH 4.5), 35% v/v MPD and 40mM calcium chloride |
