8CIU
The FERM domain of human moesin mutant H288A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 117.075, 117.075, 62.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.280 - 2.393 |
| R-factor | 0.2441 |
| Rwork | 0.238 |
| R-free | 0.30780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.150 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 2.760 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 12176 | 82705 |
| <I/σ(I)> | 21.6 | 1.6 |
| Completeness [%] | 68.6 | 29.3 |
| Redundancy | 50.9 | |
| CC(1/2) | 1.000 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 100 mM ammonium acetate, 100 mM tris, 32% propan-2-ol |
