8CIS
The FERM domain of human moesin with two bound peptides identified by phage display
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-31 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 79.714, 37.263, 81.530 |
| Unit cell angles | 90.00, 108.15, 90.00 |
Refinement procedure
| Resolution | 77.473 - 1.520 |
| Rwork | 0.182 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.727 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.500 | 1.560 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 51601 | 2581 |
| <I/σ(I)> | 13.2 | 1.4 |
| Completeness [%] | 59.7 | 12.1 |
| Redundancy | 22.4 | 25.3 |
| CC(1/2) | 0.997 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 200 mM ammonium sulphate, 100 mM sodium acetate trihydrate, 25% PEG 4000 |
