8CHY
Crystal structure of an 8-repeat consensus TPR superhelix with Zinc.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.28195 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 74.100, 74.100, 238.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.920 - 2.000 |
R-factor | 0.20568 |
Rwork | 0.204 |
R-free | 0.24869 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.546 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.920 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.172 | 8.666 |
Rpim | 0.029 | 1.427 |
Total number of observations | 981062 | 140812 |
Number of reflections | 27290 | 3862 |
<I/σ(I)> | 14.5 | 1.3 |
Completeness [%] | 100.0 | |
Redundancy | 35.9 | 36.5 |
CC(1/2) | 0.999 | 0.734 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 292 | 20mM ZnCl2 100mM NaOAc 30% MPD |