8CHY
Crystal structure of an 8-repeat consensus TPR superhelix with Zinc.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28195 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 74.100, 74.100, 238.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.920 - 2.000 |
| R-factor | 0.20568 |
| Rwork | 0.204 |
| R-free | 0.24869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.920 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.172 | 8.666 |
| Rpim | 0.029 | 1.427 |
| Total number of observations | 981062 | 140812 |
| Number of reflections | 27290 | 3862 |
| <I/σ(I)> | 14.5 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 35.9 | 36.5 |
| CC(1/2) | 0.999 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 292 | 20mM ZnCl2 100mM NaOAc 30% MPD |
