8CHY

Crystal structure of an 8-repeat consensus TPR superhelix with Zinc.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28195
Spacegroup name	P 61 2 2
Unit cell lengths	74.100, 74.100, 238.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.920 - 2.000
R-factor	0.20568
Rwork	0.204
R-free	0.24869
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.546
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.920	2.110
High resolution limit [Å]	2.000	2.000
Rmeas	0.172	8.666
Rpim	0.029	1.427
Total number of observations	981062	140812
Number of reflections	27290	3862
<I/σ(I)>	14.5	1.3
Completeness [%]	100.0
Redundancy	35.9	36.5
CC(1/2)	0.999	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	292	20mM ZnCl2 100mM NaOAc 30% MPD