8CH9
Crystal structure of arsenite oxidase from Alcaligenes faecalis (Af Aio) bound to arsenic oxyanion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.288, 109.950, 117.424 |
| Unit cell angles | 98.33, 89.97, 96.58 |
Refinement procedure
| Resolution | 65.470 - 1.430 |
| R-factor | 0.16811 |
| Rwork | 0.167 |
| R-free | 0.19459 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.171 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.474 | 1.560 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Number of reflections | 594307 | 29716 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 75.0 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.997 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% v/v PEG 6000, 0.01 M magnesium chloride |
