8CH0

Crystal structure of an 8-repeat consensus TPR superhelix with Gadolinium.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.711822
Spacegroup name	P 21 21 21
Unit cell lengths	44.228, 57.730, 76.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 1.750
R-factor	0.18005
Rwork	0.179
R-free	0.20984
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.774
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.730	1.840
High resolution limit [Å]	1.750	1.750
Rmeas	0.106	0.687
Rpim	0.031	0.207
Total number of observations	234292	32240
Number of reflections	20384	2930
<I/σ(I)>	13.2	3.1
Completeness [%]	100.0
Redundancy	11.5	11
CC(1/2)	0.997	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	292	5mM GdCl3 100 mM Tris HCl 30% MPD