8CGS
Crystal structure of arsenite oxidase from Alcaligenes faecalis (Af Aio) bound to antimony oxyanion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 |
Unit cell lengths | 90.378, 109.210, 117.342 |
Unit cell angles | 97.71, 90.06, 96.28 |
Refinement procedure
Resolution | 116.270 - 1.840 |
R-factor | 0.15536 |
Rwork | 0.154 |
R-free | 0.18891 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.954 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.470 | 1.870 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 365490 | 17998 |
<I/σ(I)> | 4.2 | |
Completeness [%] | 95.3 | |
Redundancy | 2 | |
CC(1/2) | 0.993 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 15% v/v PEG 4000, 0.1 M sodium citrate pH 5.5 and 0.2 M ammonium acetate |