8CGQ

Crystal structure of UGT708A6 with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	51.431, 72.593, 57.018
Unit cell angles	90.00, 101.69, 90.00

Refinement procedure

Resolution	44.260 - 2.040
R-factor	0.1802
Rwork	0.178
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.099
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.260	2.120
High resolution limit [Å]	2.040	2.040
Rmerge	0.108	0.925
Rmeas	0.117	1.063
Rpim	0.046	0.510
Number of reflections	25283	2028
<I/σ(I)>	10.1	1.22
Completeness [%]	96.7	77.62
Redundancy	6.4	4.1
CC(1/2)	0.998	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	PEG 3350 20% w/v, BIS-TRIS propane 100mM,