8CGM
Structure of the lipoprotein transporter LolA from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.810, 76.463, 99.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.720 - 1.700 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.22120 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.559 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.720 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.134 | 1.299 |
| Rmeas | 1.349 | |
| Rpim | 0.038 | 0.359 |
| Number of reflections | 41710 | 4084 |
| <I/σ(I)> | 20.3 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.4 | 26.9 |
| CC(1/2) | 0.999 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Na formate, 0.2 M NH4acetate, 0.2 M Na citrate tribasic, 0.2 M Na/K tartrate, 0.1 M imidazole/MES pH 6.5, 40% PEG500mme, 20% PEG20000 |
