8CEK
Succinyl-CoA Reductase from Clostridium kluyveri (SucD) with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-03-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97620 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 140.046, 190.828, 190.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.660 - 2.150 |
R-factor | 0.1983 |
Rwork | 0.198 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.585 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.440 | 2.150 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmeas | 0.215 | 2.931 |
Rpim | 0.059 | 0.966 |
Total number of observations | 2045343 | 169254 |
Number of reflections | 159239 | 21156 |
<I/σ(I)> | 8.5 | 0.6 |
Completeness [%] | 98.6 | |
Redundancy | 12.8 | 8 |
CC(1/2) | 0.998 | 0.329 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288.15 | 45 % w/v Pentaerythritol Propoxylate (5/4 PO/OH) 100 mM MES; pH 6.5 400 mM Potassium chloride |