8CEG

BAR domain protein FAM92A1 essential for mitochondrial membrane remodeling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.89998
Spacegroup name	P 1
Unit cell lengths	49.044, 54.915, 57.505
Unit cell angles	88.32, 67.40, 63.86

Refinement procedure

Resolution	28.920 - 2.030
R-factor	0.1872
Rwork	0.185
R-free	0.23000
Structure solution method	AB INITIO PHASING
RMSD bond length	0.013
RMSD bond angle	1.155
Phasing software	Arcimboldo (1.0.16)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.062
Number of reflections	30902	2177
<I/σ(I)>	11	1.7
Completeness [%]	97.4	94.2
Redundancy	3.4
CC(1/2)	0.998	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.1 M Citrate, 6% tert-butanol