8CDY
N-terminal domain of human apolipoprotein E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.567, 53.025, 72.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.591 - 1.900 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.23070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.695 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.820 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 13501 | 563 |
| <I/σ(I)> | 33.8 | |
| Completeness [%] | 93.7 | |
| Redundancy | 11.1 | |
| CC(1/2) | 1.000 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | HEPES, PEG 6000 |
