8CDT

Crystal structure of the xNup93-Nb2t VHH antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	99.968, 99.968, 30.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.720 - 1.410
R-factor	0.1593
Rwork	0.158
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.535
Data reduction software	XDS (Jan 31, 2020 BUILT=20200417)
Data scaling software	XDS (Jan 31, 2020 BUILT=20200417)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.290	43.290	1.410
High resolution limit [Å]	1.330	3.960	1.330
Rmerge	0.045	0.034	1.818
Rmeas	0.047	0.036	1.919
Number of reflections	79377	3001	12488
<I/σ(I)>	22.9
Completeness [%]	99.5
Redundancy	19.8
CC(1/2)	1.000	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293.15	0.1 M CAPS (pH 10.5), 1.2 M sodium phosphate, 0.8 M potassium phosphate, 0.2 M lithium sulfate