8CDT
Crystal structure of the xNup93-Nb2t VHH antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 99.968, 99.968, 30.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.720 - 1.410 |
R-factor | 0.1593 |
Rwork | 0.158 |
R-free | 0.18820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.535 |
Data reduction software | XDS (Jan 31, 2020 BUILT=20200417) |
Data scaling software | XDS (Jan 31, 2020 BUILT=20200417) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.290 | 43.290 | 1.410 |
High resolution limit [Å] | 1.330 | 3.960 | 1.330 |
Rmerge | 0.045 | 0.034 | 1.818 |
Rmeas | 0.047 | 0.036 | 1.919 |
Number of reflections | 79377 | 3001 | 12488 |
<I/σ(I)> | 22.9 | ||
Completeness [%] | 99.5 | ||
Redundancy | 19.8 | ||
CC(1/2) | 1.000 | 0.999 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293.15 | 0.1 M CAPS (pH 10.5), 1.2 M sodium phosphate, 0.8 M potassium phosphate, 0.2 M lithium sulfate |