8CD4
structure of HEX-1 from N. crassa crystallized in cellulo (cytosol), diffracted at 100K and resolved using CrystFEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 57.950, 57.950, 192.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.190 - 1.830 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1khi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.016 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.190 | 1.895 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 31913 | 1730 |
| <I/σ(I)> | 22.16 | |
| Completeness [%] | 99.9 | |
| Redundancy | 1382 | |
| CC(1/2) | 0.999 | 0.275 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 300 | baculovirus infected and grown in Trichoplusia ni (High Five) cells in adhesion culture (MOI 1) |
