8CD3

Crystal structure of human Scribble PDZ1 domain in complex with human TMIGD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	I 41 2 2
Unit cell lengths	53.664, 53.664, 215.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.010 - 1.900
R-factor	0.1975
Rwork	0.196
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.284
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.010	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.078
Number of reflections	13031	809
<I/σ(I)>	21.7
Completeness [%]	100.0
Redundancy	26.1
CC(1/2)	1.000	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	24% w/v PEG 1500, 20% Ethylene Glycol.