8CC5

Vibrio cholerae GbpA (LPMO domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-29
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 2
Unit cell lengths	75.333, 89.374, 47.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.542 - 1.620
Rwork	0.176
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.341
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.542	1.680
High resolution limit [Å]	1.620	1.620
Number of reflections	78744	4085
<I/σ(I)>	10.83
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.998	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Zinc Acetate 0.1 M Sodium Cacodylate pH 6.5 18% w/v PEG 8K