8CC3

Vibrio cholerae GbpA (LPMO domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 2
Unit cell lengths	74.911, 89.103, 47.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.135 - 1.128
Rwork	0.173
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.504
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.140	1.170
High resolution limit [Å]	1.128	1.130
Number of reflections	221328	11391
<I/σ(I)>	6.13
Completeness [%]	98.7
Redundancy	2.2
CC(1/2)	0.995	0.219

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Zinc Acetate 0.1 M Sodium Cacodylate pH 6.5 18% w/v PEG 8K