8CBX
Crystal Structure of Anti-Cortisol Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-04 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.873128 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 80.015, 80.015, 254.161 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.010 - 2.000 |
R-factor | 0.2241 |
Rwork | 0.223 |
R-free | 0.23880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mhe |
RMSD bond length | 0.009 |
RMSD bond angle | 1.073 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.010 | 2.074 |
High resolution limit [Å] | 2.000 | 2.002 |
Rmerge | 0.117 | 1.051 |
Number of reflections | 33324 | 3095 |
<I/σ(I)> | 13.3 | 1.85 |
Completeness [%] | 99.5 | |
Redundancy | 17.6 | |
CC(1/2) | 0.998 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 288.15 | PEG3350, citric acid |