8CBB
Structure of homodimeric luciferase from Enhygromyxa salina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-11 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.821, 73.374, 93.972 |
| Unit cell angles | 88.72, 77.93, 87.10 |
Refinement procedure
| Resolution | 91.890 - 2.710 |
| R-factor | 0.19478 |
| Rwork | 0.191 |
| R-free | 0.26877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.338 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.890 | 2.840 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.068 | 0.218 |
| Rmeas | 0.096 | 0.308 |
| Rpim | 0.068 | 0.218 |
| Total number of observations | 63062 | 8436 |
| Number of reflections | 35543 | 4681 |
| <I/σ(I)> | 4 | 2 |
| Completeness [%] | 97.0 | |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.981 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350 25%, lithium sulfate 0.2 M, BIS-TRIS 0.1 M pH 5.5 |
