8CB2
A complex of cagX and cagY components of Helicobacter pylori type IV secretion system
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9689 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.059, 196.123, 200.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.079 - 2.700 |
| Rwork | 0.252 |
| R-free | 0.29800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.080 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 30397 | 3985 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.1 | |
| CC(1/2) | 0.990 | 0.241 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.2M Calcium acetate, 0.1M Bis-tris pH 5.5 and 20% PEG 3350 |
