8C9G
Priestia megaterium mupirocin-sensitive isoleucyl-tRNA synthetase 1 complexed with mupirocin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999873570222049 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.820, 144.280, 108.370 |
Unit cell angles | 90.00, 95.64, 90.00 |
Refinement procedure
Resolution | 60.390 - 2.800 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.453 |
Data reduction software | XDS (20190806) |
Data scaling software | XSCALE (20190806) |
Phasing software | PHASER (CCP4Interface 7.0.077) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.390 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.150 | 1.288 |
Rmeas | 0.177 | 1.513 |
Rpim | 0.092 | 0.786 |
Number of reflections | 49643 | 3810 |
<I/σ(I)> | 6.95 | 0.94 |
Completeness [%] | 90.3 | 75.64 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.993 | 0.373 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Solution of 20 mg/ml wt-HMGH-PmIleRS1 with added 3 mM mupirocin was mixed in 1:1 ratio with the well solution containing: 400 - 900 mM LiCl and 10 - 20% PEG3350, 25 mM Hepes-KOH pH=7.5 at 20 oC, 50 mM NaCl. The drop volume of 2 microliters, was equilibrated towards 300 microliters of the well solution at 19 degrees. |