8C8V
Priestia megaterium mupirocin-resistant isoleucyl-tRNA synthetase 2 with a fully-resolved C-terminal tRNA-binding domain complexed with an isoleucyl-adenylate analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-17 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 108.150, 108.150, 240.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.310 - 2.200 |
R-factor | 0.1966 |
Rwork | 0.196 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.526 |
Data reduction software | XDS (20190806) |
Data scaling software | XSCALE (20190806) |
Phasing software | PHASER (CCP4Interface 7.0.077) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.310 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.076 | 1.320 |
Rmeas | 0.080 | 1.388 |
Rpim | 0.022 | 0.426 |
Number of reflections | 72948 | 5848 |
<I/σ(I)> | 26.51 | 1.66 |
Completeness [%] | 94.1 | 81.54 |
Redundancy | 12.7 | 10.4 |
CC(1/2) | 1.000 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 5 mM Ile-AMS and 16.5 mg/ml wt-HVGH-PmIleRS2 were mixed in 1:1 ratio with the well solution containing: 0.2-0.5 M Potassium tartrate, 10-20 % (w/v) PEG3350, 25 mM Hepes-KOH pH=7,5 at 20 oC, 50 mM NaCl. The drop volume of 2 microliters, was equilibrated towards 300 microliters of the well solution at 4 degrees. |