8C8R
In situ structure of the Nitrosopumilus maritimus S-layer - Composite map between C2 and C6
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum LS |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SUBTOMOGRAM AVERAGING |
Resolution (Å) | 3.5 |
Number of Particle | 1971908 |
Software | RELION,PHENIX,REFMAC,SerialEM,CTFFIND,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |