8C8F
Crystal structure of the E. coli maltodextrin-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.709, 64.961, 57.090 |
| Unit cell angles | 90.00, 101.05, 90.00 |
Refinement procedure
| Resolution | 37.760 - 1.150 |
| R-factor | 0.12498 |
| Rwork | 0.124 |
| R-free | 0.15203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.983 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.040 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 110383 | 5258 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.999 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Tris pH 7.4, 30 % PEG Smear Broad, 0.1 M NaBr and 0.1 M KSCN |
